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doi:10.1371/journal.pgen.1005843.s002(TIF) S3 Fig. Analyzed the data: RB VK AM. > > >>>> [Single] >??????????? - Brave(320kbps UPDATED) [2011. (2005) Uncoupling of unwinding from DNA synthesis implies regulation of MCM helicase by Tof1/Mrc1/Csm3 checkpoint complex. Mimura S, Yamaguchi T, Ishii S, Noro E, Katsura T, et al. Hoege C, Pfander B, Moldovan G-L, Pyrowolakis G, Jentsch S (2002) RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO. Next Thread. doi: 10.1038/sj.emboj.7600186. This model might also help explain the higher rate of HR (Fig 5A), as mrc1 strains leave more unreplicated single stranded DNA stretches at stalled replication forks [11] that require post-replicative HR. You cannot download any of those files from here.

In contrast, ctf4 mrc1 double mutants are inviable ([3,43], S7 Fig), implying that Mrc1 and Ctf4 share a Mms22-independent essential function during DNA replication. PLoS Genet 12(2): e1005843. Serial dilution of wild-type (WT), rtt101, csm3, csm3 rtt101, tof1, tof1 rtt101, tof1 rtt101 csm3 and csm3 tof1 cells were analyzed on normal growth media (YPD) with or without 0.01% and 0.02% MMS.(B) The checkpoint-defective Mrc1-AQ allele does not suppress the sensitivity of mms22 or rtt101 cells to MMS. Plates were imaged after 72 hours at 30C. This complex, composed of Rtt101, Mms1 and Mms22 in budding yeast, plays a critical role in regulating the fate of stalled DNA replication. (2009) Ctf4 coordinates the progression of helicase and DNA polymerase alpha. The plates were imaged after 48 hours of incubation at 30C. pmid:21593207 View Article PubMed/NCBI Google Scholar Download PDF Citation XML Print Print article EzReprint Share Reddit Google+ StumbleUpon Facebook LinkedIn CiteULike Mendeley PubChase Twitter Email Subject Areas ? For more information about PLOS Subject Areas, click here. PA-tagged Mms22 was immunoprecipitated from cells synchronized in S-phase and associated proteins were identified by MS-analysis.

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